Purpose
Identification and Quantitation
Method(s)
High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS)
Suggested CPT Code(s)
80364
New York State Approval Status

Approved

Known Interference(s)
Cyclopropylfentanyl [LC-MS/MS]: Crotonylfentanyl impurity
Isobutyrylfentanyl [LC-MS/MS]: beta-methylfentanyl
ortho-Fluorofentanyl [LC-MS/MS]: Ortho-fluorobutyrylfentanyl impurity
para-Fluorobutyrylfentanyl [LC-MS/MS]: meta-Fluorobutyrylfentanyl
para-Fluorofentanyl [LC-MS/MS]: Meta-fluorofentanyl
trans-3-Methylfentanyl [LC-MS/MS]: Crotonylfentanyl
Turnaround Time

5 days

Test Includes
1 Methoxyacetylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
2 4-ANPP Despropionyl fentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.1 NPS
3 THF-F Tetrahydrofuran fentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
4 meta-Methylmethoxyacetylfentanyl 3-Methylmethoxyacetylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
5 para-Methylmethoxyacetylfentanyl 4-Methylmethoxyacetylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
6 Acryl Fentanyl Acryloylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
7 para-Fluorofentanyl 4-Fluorofentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS Meta-fluorofentanyl
8 ortho-Fluorofentanyl 2-Fluorofentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS Ortho-fluorobutyrylfentanyl impurity
9 2-Furanylfentanyl Fu-F; Furanylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
10 U-47700 U-4 High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
11 U-49900 High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
12 U-51754 High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
13 Carfentanil Carfentanyl; Wildnil® High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
14 Cyclopropylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS Crotonylfentanyl impurity
15 trans-3-Methylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS Crotonylfentanyl
16 cis-3-Methylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
17 Isobutyrylfentanyl Isobutyrfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS beta-methylfentanyl
18 Butyrylfentanyl Butyrfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
19 para-Fluoroisobutyrylfentanyl 4F-Isobutyrylfentanyl; FIBF High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
20 para-Fluorobutyrylfentanyl 4F-butyryl fentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS meta-Fluorobutyrylfentanyl
21 Valeryl Fentanyl Pentylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS

Specimen Type
Serum or Plasma
Requested Volume
2 mL
Minimum Volume
0.7 mL
Special Handling
Promptly centrifuge and separate Serum or Plasma into a plastic screw capped vial using approved guidelines.
Specimen Container
Plastic container (preservative-free)

Additional Collection Instructions

Transport Temperature
Refrigerated
Light Protection
Not Required
Rejection Criteria
Received Room Temperature. Polymer gel separation tube (SST or PST).

Rejection criteria pertain to clinical specimen submissions only.

Stability
Room Temperature: 1 day(s)
Refrigerated: 14 day(s)
Frozen (-20 °C): 30 day(s)
1 Methoxyacetylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
2 4-ANPP Despropionyl fentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.1 NPS
3 THF-F Tetrahydrofuran fentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
4 meta-Methylmethoxyacetylfentanyl 3-Methylmethoxyacetylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
5 para-Methylmethoxyacetylfentanyl 4-Methylmethoxyacetylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
6 Acryl Fentanyl Acryloylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
7 para-Fluorofentanyl 4-Fluorofentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS Meta-fluorofentanyl
8 ortho-Fluorofentanyl 2-Fluorofentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS Ortho-fluorobutyrylfentanyl impurity
9 2-Furanylfentanyl Fu-F; Furanylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
10 U-47700 U-4 High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
11 U-49900 High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
12 U-51754 High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
13 Carfentanil Carfentanyl; Wildnil® High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
14 Cyclopropylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS Crotonylfentanyl impurity
15 trans-3-Methylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS Crotonylfentanyl
16 cis-3-Methylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
17 Isobutyrylfentanyl Isobutyrfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS beta-methylfentanyl
18 Butyrylfentanyl Butyrfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
19 para-Fluoroisobutyrylfentanyl 4F-Isobutyrylfentanyl; FIBF High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS
20 para-Fluorobutyrylfentanyl 4F-butyryl fentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS meta-Fluorobutyrylfentanyl
21 Valeryl Fentanyl Pentylfentanyl High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 0.05 NPS

Interface Map

LOINC® Information