Purpose
Exclusion Screen
Method(s)
High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS)
Suggested CPT Code(s)
80307
New York State Approval Status

Approved

Turnaround Time

4 days (If Positive: 7 days)

Test Includes
1 Tramadol Ultram®; Ultrex® High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 10 Analgesic, Anti-Inflammatory
2 O-Desmethyltramadol Tramadol Metabolite High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 10 Analgesic, Anti-Inflammatory

Reflex Tests
Test Code Test Name
5626SP Tramadol and Metabolite Confirmation, Serum/Plasma
Specimen Type
Serum or Plasma
Requested Volume
2 mL
Minimum Volume
0.9 mL
Special Handling
Serum: Collect sample in Red top tube
Plasma: Collect sample in Lavender top tube (EDTA) or Pink top tube.
Peak serum levels are recommended when monitoring patients because the level in the blood drops so rapidly that many negative results are found at the trough. The peak occurs at 40 to 90 minutes post dose. Promptly centrifuge and separate Serum or Plasma into a plastic screw capped vial using approved guidelines.
Specimen Container
Plastic container (preservative-free)

Additional Collection Instructions

Transport Temperature
Refrigerated
Light Protection
Not Required
Rejection Criteria
Polymer gel separation tube (SST or PST).

Rejection criteria pertain to clinical specimen submissions only.

Stability
Room Temperature: 14 day(s)
Refrigerated: 14 day(s)
Frozen (-20 °C): 11 month(s)
1 Tramadol Ultram®; Ultrex® High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 10 Analgesic, Anti-Inflammatory
2 O-Desmethyltramadol Tramadol Metabolite High Performance Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS) ng/mL 10 Analgesic, Anti-Inflammatory

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