Vitreous glucose levels decrease rapidly after death in vivo. This decrease is time and temperature-dependent, with a delay in loss facilitated by cold temperatures. The in vivo loss of glucose is reported to stop at approximately 18 hours post-death. Additionally, NMS studies have demonstrated a continued in vitro loss of glucose in vitreous when specimens are maintained at room temperature or refrigerated. No in vitro loss occurs when specimens are maintained frozen (-20°C) for up to 30 days.